Biocomposites of covalently linked glucose oxidase on carbon nanotubes for glucose biosensor

The formation of covalently linked composites of multi–walled carbon nanotubes (MWCNT) and glucose oxidase (GOD) with high-function density for use as a biosensing interface is described. The reaction intermediates and the final product were characterized by using FT–IR spectroscopy, and the MWCNT-coated GOD nanocomposites were examined by atomic force microscopy (AFM) and transmission electron microscopy (TEM). Interestingly, it was found that the GOD–MWCNT composites are highly water soluble. Electrochemical characterization of the GOD–MWCNT composites that were modified on a glassy carbon electrode shows that the covalently linked GOD retains its bioactivity and can specifically catalyze the oxidation of glucose. The oxidation current shows a linear dependence on the glucose concentration in the solution in the range of 0.5–40 mM with a detection limit of 30 μM and a detection sensitivity of 11.3 μA/mMcm². The present method may provide a way to synthesize MWCNT related composites with other biomolecules and for the construction of enzymatic reaction-based biofuel cells and biosensors. Supported by grants from the National Natural Science Foundation of China (NSFC, No. 20125515; 90206037; 20375016) and the Natural Science Foundation of Jiangsu Province (Grant No. BK 2004210)     

钛酸盐功能材料的研究与应用

本文综述了钛酸盐功能材料的各种性质、合成方法及用途。指出关于新型钛酸盐产品的制备、新性能的发现及寻求新的应用领域是一项值得深入研究的课题。     

温敏性聚合物复合体系低临界溶解温度的研究

研究了温敏性N-异丙基丙烯酰胺的均聚物(PNIPAm)及其共聚物P(NIPAm-co-KYD)与十六烷基三甲基氯化铵(CTAC)、乙二胺四乙酸(EDTA)、盐等复配体系的低临界溶解温度(LCST)的变化规律。单因素复配体系中,wEDTA为0.1%时,体系LCST从33℃降低到25℃,增大到0.2%时,LCST下降趋于缓慢;wCTAC在0.5%-3.0%范围内,LCST先上升后下降,但wCTAC在0.5%-1.0%内相转变很不明显,超过1.5%后相转变又趋于明显;而无机盐能使体系LCST线性下降;多因素复配体系中LCST变化较缓和,易于控制且相转变现象明显。     

MOLECULAR ENGINEERING STUDIES ON NONTHROMBOGENIC BIOMATERIALS——A NOVEL CLASS OF NONTHROMBOGENIC BIOMATERIALS WITH ZWITTERIONIC STRUCTURE OF CARBOXYBETAINES

N,N-dimethyl-N-methacryloyloxyethyl-N-carboxyethyl ammonium (DMMCA) was graft-copolymerized onto the surface of segmented poly(ether urethane) (SPEU) and PE film. The carboxybetaine structure on SPEU and PE film surfaces was confirmed by ATR-FTIR, XPS and water contact angle measurements. Through the experiments with platelet adhesion and protein adhesion assay in vitro, the two materials studied, including poly-DMMCA gel, all show excellent nonthrombogenicity. This confirms once again that the zwitterionic molecular structure on the surfaces of materials is essential for improving their nonthrombogenicity and biocompatibility.     

希土烯丙基化合物的合成——Ⅱ.镥、钆的烯丙基化合物的合成

本文合成了两个新的对空气极敏感的希土烯丙基化合物,由无水希土氯化物与希丙基锂在四氢呋喃—乙醚中反应制得。产物经元素分析、红外光谱等确定其组成为:Li₂Ln(C₃H₅)₅·3Dioxane(Ln=Gd,Lu)。     

瑞香素稀土配合物的合成和性质

自六十年代以来陆续发现稀土化合物具有一系列特殊的药效作用 ,使稀土元素在药物上的应用展现出广阔前景。目前 ,已有不少稀土化合物应用于临床 ,如抗凝血药物 3 磺酸异烟酸钕、左旋糖酸镨、钕混合物 ;消炎药水杨酸钐等。合成具有生物活性配体的稀土配合物是寻找有效稀土药物的一条途径。瑞香素是一具有抗菌、抗炎、抗凝血等生物活性的香豆素类化合物 ,其分子结构为 :稀土与香豆素类的配合物的合成与性质研究己有不少报道[1,2 ] 。为了进一步研究这类配合物的性质和生物活性 ,我们合成了六种瑞香素稀土配合物。1　实验部分1 .1　仪器及试剂…     

接枝共聚物氯化聚乙烯—苯乙烯对聚苯乙烯的共混改性

用氯化聚乙烯接枝苯乙烯共聚物（ＣＰＥ－ｇ－Ｓｔ）和氯化聚乙烯（ＣＰＥ）对聚苯乙烯（ＰＳ）进行共混改性。当ＣＰＥ含量为２５％时，用ＣＰＥ－ｇＳｔ改性的共混物的冲击强度为１８．５ｋＪ．ｍ＾－＾２，是用ＣＰＥ改性的共混物冲击强度的２．１倍；其拉伸强度不低于３４ＭＰａ。     

Tris(2,2''''-bipyridyl)ruthenium(Ⅱ) electrochemiluminescence (ECL) enhanced by rutin on platinum electrode

Ru(bpy)_(3~(2 )) electrochemiluminescence (ECL) was applied to determination of rutin. ECL intensity of Ru(bpy)_(3~(2 ))could be enhanced in the presence of rutin in basic solution on platinum electrode. At pH 9.9, light emission intensity was found to be linear with rutin in the range of 1-50 mmol/L.     

有机硅氨基酸衍生物的研究

本文利用含氢有机硅化合物与含双键的氨基酸酯的加成反应,合成了5种新的有机硅氨基酸衍生物。介绍了一种合成N-烷基取代氨基酸酯的新方法。对新化合物进行了元素和光谱分析鉴定。     

胶原纤维固化黑荆树单宁吸附V(V)的研究

研究了胶原纤维固化黑荆树单宁对V(V)的吸附。采用不同温度、pH值等条件进行吸附研究,并进一步探讨了固化黑荆树单宁的吸附动力学和吸附柱动力学及其吸附机理。结果表明,该材料对V(V)的吸附平衡符合Freund lich方程,温度对吸附平衡的影响不明显;吸附动力学可用拟二级速度方程来描述,该材料同时具有良好的柱动力学特性;V(V)的吸附过程可能存在三个反应,即V(V)与吸附剂之间发生氧化还原反应生成V(IV),V(IV)和-COOH之间发生离子交换反应,以及V(IV)与单宁的邻位羟基发生螯合。